Replication
We have established a longstanding collaboration with the O’Donnell Lab to study the eukaryotic replisome, a multi-component macromolecular machine responsible for genome replication. Our approach—single-molecule observation combined with in vitro reconstitution—offers the best opportunity to dissect the intricate dynamics of individual constituents within this extraordinarily sophisticated machinery.

Smc5/6’s DNA binding capacities stabilize branched DNA structures

Origin recognition complex harbors an intrinsic nucleosome remodeling activity

Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
